Flavobacterium sinopsychrotolerans sp. nov., isolated from a glacier

An aerobic, Gram-negative, yellow-pigmented bacterial strain, designated 0533(T), was isolated from frozen soil from the China No. 1 glacier. Phylogenetic analysis of the 16S rRNA gene sequence demonstrated that strain 0533(T) was a member of the genus Flavobacterium and exhibited 97.1-98.7% 16S rRNA sequence similarity with its nearest phylogenetic neighbours. Strain 0533(T) exhibited phenotypic and chemotaxonomic characteristics common to the genus Flavobacterium: menaquinone-6 (MK-6) was the predominant quinone and iso-C-15:0, C-17:1 omega 6(C), anteiso-C-15:0, iso-C-15:0 3-OH, C-15:1 omega 6(C), iso-C-16:0 3-OH, summed feature 3 (comprising C-16:1 omega 7C and/or C-16:1 omega 6(C)), iso-C-15:1 G and iso-C-17:0 3-OH were the major fatty acids (>5%). The DNA G+C content was 32.5 mol%. On the basis of phenotypic and genotypic data, a novel species, Flavobacterium sinopsychrotolerans sp. nov., is proposed. The type strain is 0533(T) (=CGMCC 1.8704(T) =JCM 16398(T)).