Stakelama pacifica gen. nov., sp nov., a new member of the family Sphingomonadaceae isolated from the Pacific Ocean

A Gram reaction-negative, weakly motile, non-spore-forming, rod-shaped, aerobic bacterium designated strain JLT832(T). was isolated from surface water of the central Pacific Ocean and formed yellow colonies on rich organic (RO) medium. The strain was oxidase-negative and catalase-positive. Acid was produced from mannitol, glucose, sucrose, lactose, sorbitol, maltose, (+)-trehalose and D-fructose. No acid was produced from D-(+)-xylose. The major cellular fatty acids of strain JLT832(T) were C-18:1 omega 7c, C-14:0 2-OH and C-16:0. The major polar lipids were sphingoglycolipid, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. Ubiquinone-10 and spermidine were present as the major quinone and polyamine, respectively. The genomic DNA G + C content of strain JLT832(T) was 66.0 +/- 0.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences indicated that the new isolate formed a tight branch within the family Sphingomonadaceae but was clearly separate from established genera in this family. 1 The sequence similarities between the new isolate and type strains of established genera ranged from 90.5 to 94.9%. Based on these data, strain JLT832(T) constitutes a novel genus and species, for which the name Stakelama pacifica gen. nov., sp. nov. is proposed. The type strain of Stakelama pacifica is JLT832(T) (=CGMCC 1.7294(T) =LMG 24686(T)).