Marisediminicola antarctica gen. nov., sp. nov., an actinobacterium isolated from the Antarctic

Strain ZS314(T) was isolated from a sandy intertidal sediment sample collected from the coastal area off the Chinese Antarctic Zhongshan Station, east Antarctica (69 degrees 22' 13 '' S 76 degrees 21' 41 '' E). The cells were Gram-positive, motile, short rods. The temperature range for growth was 0-26 degrees C and the pH for growth ranged from 5 to 10, with optimum growth occurring within the temperature range 18-23 degrees C and pH range 6.0-8.0. Growth occurred in the presence of 0-6% (w/v) NaCl, with optimum growth occurring in the presence of 2-4% (w/v) NaCl. Strain ZS314(T) had MK-10 as the major menaquinone and anteiso-C-15:0, iso-C-16:0 and anteiso-C-17:0 as major fatty acids. The cell-wall peptidoglycan type was B2 beta with ornithine as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol. The genomic DNA G+C content was approximately 67 mol%. Phylogenetic analysis based on 16S rRNA gene sequence similarity showed that strain ZS314(T) represents a new lineage in the family Microbacteriaceae. On the basis of the phylogenetic analyses and phenotypic characteristics, a new genus, namely Marisediminicola gen. nov., is proposed, harbouring the novel species Marisediminicola antarctica sp. nov. with the type strain ZS314(T) (=DSM 22350(T) =CCTCC AB 209077(T)).