Sciscionella marina gen. nov., sp nov., a marine actinomycete isolated from a sediment in the northern South China Sea

The taxonomic position of an actinomycete, designated SCSIO 00231(T), isolated from a sediment sample collected from the northern South China Sea, was determined by using a polyphasic approach. The organism formed fragmented substrate hyphae and sparse aerial mycelium on modified ISP 2 medium. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain SCSIO 00231(T) fell into the family Pseudonocardiaceae, in which it formed a distinct lineage and was loosely associated with Thermocrispum municipale DSM 44069(T), with 93% similarity. The other closest phylogenetic neighbours were Saccharopolyspora erythraea NRRL 2338(T) (92.6% similarity) and Amycolatopsis sacchari DSM 44468 (T) (93.1% similarity). The isolate had cell-wall type IV (meso-diaminopimelic acid and whole-cell sugars arabinose, galactose and glucose) and phospholipid type Ill. The predominant menaquinone was MK-9(H-4)The G + C content of the genomic DNA was 69 mol%. Based on these data, strain SCSIO 00231(T) can be readily distinguished from previously described organisms and represents a new genus within the family Pseudonocardiaceae. The name Sciscionella gen. nov. is proposed, with the novel species Sciscionella marina sp. nov. The type strain of Sciscionella marina is SCSIO 00231(T) (=KCTC 19433(T) =CCTCC AA208009(T)).