Microbacterium soli sp nov., an α-glucosidase-producing bacterium isolated from soil of a ginseng field

Five Gram-type-positive, aerobic, rod-shaped, non-motile strains of Microbacterium (DCY 17(T), Ms1, Ms2, Ms3 and Ms4) were isolated from soil from a ginseng field in Daejeon, South Korea. On the basis of 16S rRNA gene sequence similarity, these strains were shown to be related to Microbacterium esteraromaticum DSM 8609(T) (96.1%), M. xylanilyticum DSM 16914(T) (96.0%), M. aquimaris JS54-2(T) (95.6%), M. insulae DS-66(T) (95.5%), M. ketosireducens IFO 14548(T) (95.5%) and M. arabinogalactanolyticum DSM 8611(T) (95.4%). Chemotaxonomic data revealed that the type strain, DCY 17T, possesses menaquinones MK-12, MK-11 and MK-13 and the predominant fatty acids C-15:0 anteiso (32.5%), C-15:0 iso (27.5%), C-16:0 iso (17.0%), C-17:0 anteiso (13.2%), C-17:0 iso (6.1%) and C-14:0 iso (2.1%). The DNA G+C content of strain DCY 17(T) is 70.2 mol% and those of strains Ms1 to Ms4 are in the range 68.9-73.5 mol%. The physiological and biochemical tests suggested that these strains represent a novel species. Based on these data, DCY 17(T) (=KCTC 19237(T) =LMG 24010(T)) is classified as the type strain of a novel Microbacterium species, for which the name Microbacterium soli sp. nov. is proposed.