Nocardioides basaltis sp nov., isolated from black beach sand

A novel Gram-positive, aerobic, short-rod-shaped bacterium, designated strain J112(T), was isolated from black sand collected from Soesoggak, Jeju Island, Korea. The strain was found to be oxidase-negative and catalase-positive. Cells grew at 10-37 degrees C, at pH 5.5-8.0 and with 1-10% NaCl. Growth occurred on marine agar but not on R2A or trypticase soy agar. A phylogenetic analysis based on 16S rRNA gene sequences showed that the strain belongs to the radiation of the genus Nocardioides. Strain J112(T) shared the highest 16S rRNA gene sequence similarities with Nocardioides marinisabuli SBS-12(T) (99.2%), Nocardioides terrigena DS-17(T) (97.3%), Nocardioides kribbensis KCTC 19038(T) (97.1%) and type strains of other Nocardioides species with validly published names (< 97%). The DNA-DNA hybridization values between strain J112(T) and the three most closely related strains were low enough to justify the assignment of this strain to a novel species. On the basis of these phenotypic, phylogenetic and chemotaxonomic data, strain J112(T) represents a novel species of the genus Nocardioides, for which the name Nocardioides basaltis sp. nov. is proposed. The type strain is J112(T) (=KCTC 19365(T)=JCM 14945(T)).