Frondihabitans peucedani sp nov., an actinobacterium isolated from rhizosphere soil, and emended description of the genus Frondihabitans Greene et al. 2009

A novel actinobacterium, designated RS-15(T), was isolated from rhizosphere soil of a plant (Peucedanum japonicum Thunb.) on Mara Island, Jeju, Republic of Korea. Cells were strictly aerobic, Gram-stain-positive, oxidase-negative, catalase-positive, non-motile, irregular, short rods. The peptidoglycan in the cell wall was of the B2 beta type with ornithine as the diagnostic diamino acid. The major menaquinone was MK-8; small amounts of MK-9 and MK-7 were also present. The polar lipids contained phosphatidylglycerol, five unknown glycolipids, three unknown phospholipids and two unknown lipids. Mycolic acids were not present. The predominant fatty acids were summed feature 7 (one or more of C-18:1 omega 9c, C-18:1 omega 12t and C-18:1 omega 7c), anteiso-C-15:0 and C-14:0 2-OH. The DNA G+C content was 68.3 mol%. 16S rRNA gene sequence analysis showed that strain RS-15T belonged to the family Microbacteriaceae and formed a tight phylogenetic cluster with Frondihabitans australicus E1HC-02(T) (98.5% sequence similarity). The DNA DNA relatedness between strain RS-15T and Frondihabitans australicus DSM 17894(T) was 17.2% (17.1%, duplicate measurements). On the basis of the physiological, chemotaxonomic and DNA DNA hybridization data, strain RS-15T is considered to represent a novel species of the genus Frondihabitans, for which the name Frondihabitans peucedani sp. nov. is proposed. The type strain is RS-15(T) (=KCTC 13435(T) =DSM 22180(T)). An emended description of the genus Frondihabitans is also presented.