Kaistia terrae sp nov., isolated from a wetland in Korea

An ivory-coloured bacterium, designated strain 5YN7-3(T), was isolated from a wetland, Yongneup, Korea. Cells of the strain were aerobic, Gram-stain-negative, non-motile and short rods. 16S rRNA gene sequence analysis demonstrated that strain 5YN7-3(T) belongs to the order Rhizobiales of the class Alpha proteobacteria and is closely related to Kaistia soli 5YN9-8(T) (97.8%), Kaistia granuli Ko04(T) (97.6%) and Kaistia adipata Chj404(T) (97.4%). Strain 5YN7-3(T) showed DNA-DNA hybridization values of 28, 22 and 35% with K. granuli Ko04(T), K. soli 5YN9-8(T) and K. adipata Chj404(T), respectively. The major fatty acids were C-18:1 omega 7C (51.2 CV), C-19:0 cyclo omega 8c (25.0%), C-18:0 (12.9 %) and C-16:0 (10.8 %) (>100/0 of total fatty acids). Ubiquinone-10 was the major isoprenoid quinone and the DNA G+C content was 66.5 molok. The phenotypic characteristics in combination with 16S rRNA gene sequence analysis and DNA-DNA hybridization data clearly define strain 5YN7-3(T) as a novel species of the genus Kaistia, for which the name Kaistia terrae sp. nov. is proposed. The type strain is 5YN7-3(T) (=KACC 12910(T) =DSM 21341(T)).