Tenacibaculum soleae sp nov., isolated from diseased sole (Solea senegalensis Kaup)

A novel Gram-negative, rod-shaped, gliding bacterial strain designated LL04 12.1.7(T) was isolated from diseased sole (Solea senegalensis Kaup) in Galicia, Spain. Colonies were yellow-pigmented with uneven edges and did not adhere to the agar. The DNA G + C content of the strain was 29.8 mol%. 16S rRNA gene sequence similarity analysis indicated that strain LL04 12.1.7(T) is a member of the genus Tenacibaculum in the family Flavobacteriaceae. Sequence similarities between the isolate and the type strains of other members of the genus were 96.7-94.8 %. The major fatty acids (>10% of total fatty acids) were iso-C-15:0 (23.1 %), iso-C-15:0 3-OH (10.6%), C-15:1 omega 6c (12.2%) and summed feature 3 (comprising C-16:1 omega 7c and/or iso-C-15:0 2-OH, 11.0 %). Genotypic and phenotypic data distinguished strain LL04 12.1.7(T) from the 11 recognized Tenacibaculum species, indicating that it represents a novel species, for which the name Tenacibaculum soleae sp. nov. is proposed. The type strain is strain LL04 12.1.7(T) (=CECT 7292(T) =NCIMB 14368(T)).