Microbacterium ginsengisoli sp nov., a β-glucosidase-producing bacterium isolated from soil of a ginseng field

Strain Gsoil 259(T), a beta-glucosidase-producing bacterium, was isolated from a soil sample from a ginseng field in the Republic of Korea and characterized in order to determine its taxonomic position. Cells were Gram-positive, heterotrophic, strictly aerobic, non-motile short rods. 16S rRNA gene sequence analysis revealed that strain Gsoil 259(T) belonged to the genus Microbacterium and was closely related to Microbacterium arborescens IFO 3750(T) (98.5%) and Microbacterium imperiale IFO 12610(T) (97.9%). However, it has low values for DNA-DNA relatedness with the above strains (20.7 and 17.5 %, respectively). Strain Gsoil 259(T) possessed chemotaxonomic markers that were consistent with classification in the genus Microbacterium, i.e. MK-11 and MK-12 were the major menaquinones and anteiso-C-17:0, anteiso-C-15:0 and iso-C-16:0 were the predominant cellular fatty acids. The DNA G + C content was 69.4 mol%. The cell-wall sugar was rhamnose and the diamino acid in the cell-wall pepticloglycan was ornithine. On the basis of data from this polyphasic study, strain Gsoil 259(T) represents a novel species of the genus Microbacterium, for which the name Microbacterium ginsengisoli sp. nov. is proposed. The type strain is Gsoil 259(T) (=KCTC 19189(T) =DSM 18659(T)).