Klugiella xanthotipulae gen. nov., sp nov., a novel member of the family Microbacteriaceae

An actinobacterium, designated strain 44C3(T), was isolated in Michigan, USA, from the hindgut of the larvae of Tipula abotominalis, an aquatic crane fly, and was subjected to a polyphasic taxonomic investigation. Phylogenetic analysis based on 11 rRNA gene sequences revealed that the strain represented a separate clade within the family Microbacteriaceae. It showed highest 11 rRNA gene sequence similarity with Cryobacterium psychrotolerans 0549(T) (96.5 %). Strain 44C3(T) had a novel B-type peptidoglycan. The peptidoglycan contained the diamino, acid lysine, the peptide Gly-D-Glu was detected in the partial hydrolysate and alanine was the N terminus of the interpeptide bridge. No other amino acids found in other B-type peptidoglycans (including diaminobutyric acid, ornithine, homoserine and hydroxyglutamic acid) could be detected. The major menaquinones were MK-12 and MK-11, the major fatty acids were ai-C-15:0, ai-C-17:0 and iC(16:0) and the DNA G + C content was 60.9 mol%. Analysis of the chemotaxonomic and phylogenetic data suggested that strain 44C3T represented a novel species of a new genus within the family Microbacteriaceae, for which the name Klugiella xanthotipulae gen. nov., sp. nov. is proposed. The type strain of Klugiella xanthotipulae is 44C3(T) (=DSM 18031(T) =ATCC BAA-1524(T)).