期刊
MOLECULAR IMMUNOLOGY
卷 66, 期 2, 页码 164-170出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.molimm.2015.02.029
关键词
Antibodies; Complement system; Leukemia; Lymphoma; C4d; C5b
资金
- Swedish Cancer Society
- Swedish Research Council [K2012-66X-14928-09-5, K2010-80X-21514-01-4]
- ALF
- Foundation of Osterlund
- Foundation of Greta and Johan Kock
- Foundation of Malmo Cancer
- Foundation of Knut and Alice Wallenberg
- Foundation of Inga-Britt and Arne Lundberg
- Skane University Hospital
An emerging number of diseases and therapeutic approaches with defined involvement of the complement system justify a need for specific markers reflecting activation of particular effector arms of the complement cascade. Measurement of such soluble markers in circulation is a challenge since the specificity of antibodies must be limited to activated complement fragments but not predominant and ubiquitous parental molecules. Existing assays for the measurement of soluble, activated complement proteins are based on the detection of conformational neoepitopes. We tested an alternative approach based on detection of short linear neoepitopes exposed at the cleavage sites after activation of the actual complement component. Obtained antibodies reactive to C4d and C5b fragments enabled us to set up highly specific sandwich ELISAs, which ensured trustful measurements without false positive readouts characteristic for some of the widely used assays. (C) 2015 Elsevier Ltd. All rights reserved.
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