期刊
MOLECULAR ECOLOGY RESOURCES
卷 16, 期 1, 页码 176-182出版社
WILEY
DOI: 10.1111/1755-0998.12428
关键词
biodiversity; next-generation sequencing; PCR errors; sequence analysis; taxonomic annotation
资金
- Agence Nationale de la Recherche (ANR) through the METABAR
DNA metabarcoding offers new perspectives in biodiversity research. This recently developed approach to ecosystem study relies heavily on the use of next-generation sequencing (NGS) and thus calls upon the ability to deal with huge sequence data sets. The OBITOOLS package satisfies this requirement thanks to a set of programs specifically designed for analysing NGS data in a DNA metabarcoding context. Their capacity to filter and edit sequences while taking into account taxonomic annotation helps to set up tailor-made analysis pipelines for a broad range of DNA metabarcoding applications, including biodiversity surveys or diet analyses. The OBITOOLS package is distributed as an open source software available on the following website: http://metabarcoding.org/obitools. A Galaxy wrapper is available on the GenOuest core facility toolshed: http://toolshed.genouest.org.
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