期刊
MOLECULAR ECOLOGY RESOURCES
卷 15, 期 5, 页码 1091-1101出版社
WILEY
DOI: 10.1111/1755-0998.12379
关键词
Brassicaceae; genebanks; genetic resources; germplasm collections; Illumina Infinium SNP array; molecular genotyping
资金
- Australian Research Council [LP0882095, LP0883462, LP0989200, DE120 100668, LP110100200, LP130100061, LP130100 925, FT130100604, DP0985953]
- Australian Research Council [LP130100061] Funding Source: Australian Research Council
Germplasm collections provide an extremely valuable resource for breeders and researchers. However, misclassification of accessions by species often hinders the effective use of these collections. We propose that use of high-throughput genotyping tools can provide a fast, efficient and cost-effective way of confirming species in germplasm collections, as well as providing valuable genetic diversity data. We genotyped 180 Brassicaceae samples sourced from the Australian Grains Genebank across the recently released Illumina Infinium Brassica 60K SNP array. Of these, 76 were provided on the basis of suspected misclassification and another 104 were sourced independently from the germplasm collection. Presence of the A- and C-genomes combined with principle components analysis clearly separated Brassicarapa, B.oleracea, B.napus, B.carinata and B.juncea samples into distinct species groups. Several lines were further validated using chromosome counts. Overall, 18% of samples (32/180) were misclassified on the basis of species. Within these 180 samples, 23/76 (30%) supplied on the basis of suspected misclassification were misclassified, and 9/105 (9%) of the samples randomly sourced from the Australian Grains Genebank were misclassified. Surprisingly, several individuals were also found to be the product of interspecific hybridization events. The SNP (single nucleotide polymorphism) array proved effective at confirming species, and provided useful information related to genetic diversity. As similar genomic resources become available for different crops, high-throughput molecular genotyping will offer an efficient and cost-effective method to screen germplasm collections worldwide, facilitating more effective use of these valuable resources by breeders and researchers.
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