期刊
MOLECULAR CELL
卷 58, 期 2, 页码 216-231出版社
CELL PRESS
DOI: 10.1016/j.molcel.2015.02.023
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资金
- Intramural NIH HHS Funding Source: Medline
- NCI NIH HHS [P30 CA023108] Funding Source: Medline
- NHGRI NIH HHS [R01 HG005119] Funding Source: Medline
- NIDDK NIH HHS [DK015602] Funding Source: Medline
- NIGMS NIH HHS [R01 GM069462, R01 GM035463] Funding Source: Medline
Chromosomes of metazoan organisms are partitioned in the interphase nucleus into discrete topologically associating domains (TADs). Borders between TADs are formed in regions containing active genes and clusters of architectural protein binding sites. The transcription of most genes is repressed after temperature stress in Drosophila. Here we show that temperature stress induces relocalization of architectural proteins from TAD borders to inside TADs, and this is accompanied by a dramatic rearrangement in the 3D organization of the nucleus. TAD border strength declines, allowing for an increase in long-distance inter-TAD interactions. Similar but quantitatively weaker effects are observed upon inhibition of transcription or depletion of individual architectural proteins. Heat shock-induced inter-TAD interactions result in increased contacts among enhancers and promoters of silenced genes, which recruit Pc and form Pc bodies in the nucleolus. These results suggest that the TAD organization of metazoan genomes is plastic and can be reconfigured quickly.
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