期刊
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
卷 90, 期 12, 页码 1169-1178出版社
TAYLOR & FRANCIS LTD
DOI: 10.3109/09553002.2014.930536
关键词
Protein damage; radiosensitivity; protein biochemistry
资金
- Defense Medical Research and Development Program [D61 I 10 J7 62]
Purpose : Protein oxidation in response to radiation results in DNA damage, endoplasmic reticulum stress/unfolded protein response, cell cycle arrest, cell death and senescence. The liver, a relatively radiosensitive organ, undergoes measurable alterations in metabolic functions following irradiation. Accordingly, we investigated radiation-induced changes in liver metabolism and alterations in protein oxidation. Materials and methods : C57BL/6 mice were sham irradiated or exposed to 8.5 Gy Co-60 (0.6 Gy/min) total body irradiation. Metabolites and metabolic enzymes in the blood and liver tissue were analyzed. Two-dimensional gel electrophoresis and OxyBlot (TM) were used to detect carbonylated proteins that were then identified by peptide mass fingerprinting. Results : Analysis of serum metabolites revealed elevated glucose, bilirubin, lactate dehydrogenase (LDH), high-density lipoprotein, and aspartate aminotransferase within 24-72 h post irradiation. Liver tissue LDH and alkaline phosphatase activities were elevated 24-72 h post irradiation. OxyBlotting revealed that the hepatic proteome contains baseline protein carbonylation. Radiation exposure increased carbonylation of specific liver proteins including carbonic anhydrase 1, alpha-enolase, and regucalcin. Conclusions : 8.5 Gy irradiation resulted in distinct metabolic alterations in hepatic functions. Coincident with these changes, radiation induced the carbonylation of specific liver enzymes. The oxidation of liver enzymes may underlie some radiation-induced alterations in hepatic function.
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