期刊
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
卷 87, 期 10, 页码 1023-1032出版社
INFORMA HEALTHCARE
DOI: 10.3109/09553002.2011.584941
关键词
PC12 cells; plutonium polymer; molecular plutonium; X-ray fluorescence microscopy
资金
- Office of Basic Energy Sciences, U.S. Department of Energy
- University of Chicago
- Department of Energy [DE.AC02-06CHI1357]
- U.S. Department of Energy [DE-FG02-04ER63920, DE-FG02-05ER64086]
Purpose: To study the cellular responses to molecular and polymeric forms of plutonium using PC12 cells derived from a rat pheochromocytoma. Materials and methods: Serum starved PC12 cells were exposed to polymeric and molecular forms of plutonium for 3 h. Cells were washed with 10 mM ethylene glycol tetraacetic acid (EGTA), 100 mM NaCl at pH 7.4 to remove surface sorbed plutonium. Localization of plutonium in individual cell was quantitatively analyzed by synchrotron X-ray fluorescence (XRF) microscopy. Results: Molecular plutonium complexes introduced to cell growth media in the form of nitrilotriacetic acid (NTA), citrate, or transferrin complexes were taken up by PC12 cells, and mostly colocalized with iron within the cells. Aged polymeric plutonium prepared separately was not internalized by PC12 cells but it was always found on the cell surface as big agglomerates; however, polymeric plutonium formed in situ was mostly found within the cells as agglomerates. Conclusions: PC12 cells can differentiate molecular and polymeric forms of plutonium. Molecular plutonium is taken up by PC12 cells and mostly co-localizes with iron but aged polymeric plutonium is not internalized by the cells.
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