β-Catenin-Gli1 interaction regulates proliferation and tumor growth in medulloblastoma

Background: The Wnt/beta-catenin and the Hedgehog (Hh) pathway interact in various cell types while eliciting opposing or synergistic cellular effects. Both pathways are known as exclusive drivers of two distinct molecular subtypes of medulloblastoma (MB). In sonic hedgehog (Shh)-driven MB, activation of Wnt signaling has been shown to suppress tumor growth by either beta-catenin-dependent or -independent inhibition of Shh signaling. However, mechanistic insight in how beta-catenin inhibits the Hh pathway is not known. Findings: Here we show that beta-catenin stabilization by the glycogen synthase kinase 3 inhibitor lithium chloride (LiCI) reduced growth of primary hedgehog driven MB tumor spheres from patched heterozygous mice (Ptch') in vitro. Liel treatment of MB spheres down regulated the Hh target Glil, whereas the repressive Gli3 protein (Gli3R) was increased. Mechanistically, we show by co-immunoprecipitation and proximity ligation assay that stabilized beta-catenin physically interacts with Gli1, leading to Gli1 sequestration and inhibition of its transcriptional activity. Reduction of Hh signaling upon Liel stimulation resulted in reduced proliferation, sphere self renewal, a G2/M arrest and induction of a senescent like state, indicated by p21 upregulation and by increased staining of senescence associated beta-galactosidase (SA-betaGal). Moreover, Liel treatment of subcutaneously transplanted MB cells significantly reduced tumor initiation defined as tumor take. Although tumor progression was similar, LiCI-treated tumors showed decreased mitotic figures and phospho-histone H3 staining. Conclusion: We propose that beta-catenin stabilization increases its physical interaction with Gli1, leading to Glil degradation and inhibition of Hh signaling, thereby promoting tumor cell senescence and suppression of tumor take in mice.