4.6 Article

Association mapping of seed quality traits in Brassica napus L. using GWAS and candidate QTL approaches

期刊

MOLECULAR BREEDING
卷 35, 期 6, 页码 -

出版社

SPRINGER
DOI: 10.1007/s11032-015-0340-3

关键词

Association mapping; Brassica napus; Seed quality; SNP; Glucosinolate; Hemicellulose

资金

  1. Fondecyt [1100732]
  2. Proyecto Fortalecimiento [R13F1001]
  3. Comision Nacional de Investigacion Cientifica y Tecnologica (CONICYT) Regional Program
  4. Araucania Regional Government [CGNA/R10C1001]
  5. Becas de Magister Nacional-CONICYT [22121770]

向作者/读者索取更多资源

Single nucleotide polymorphisms (SNPs) have rapidly become the molecular marker of choice in plant and animal association mapping (AM) studies. In this work, a genome-wide association study (GWAS) and candidate quantitative trait loci (cQTL) approaches were used to identify SNP markers associated with seed quality traits, in a Brassica napus L. association panel composed of 89 adapted winter oilseed rape accessions. Six seed quality traits (oil and protein content, linolenic acid, total glucosinolates, hemicellulose and cellulose content) were evaluated in two different locations for two seasons. For GWAS, 4025 SNP markers evenly distributed along the B. napus genome were genotyped using a 6K Illumina array platform. For cQTL, 100 SNP markers previously discovered in genomic regions underlying seed quality QTL were genotyped using a competitive allele-specific PCR (KASPar). Analysis of the population structure revealed the presence of two weakly differentiated subpopulations (F-ST = 0.037), with 82 % of the pairwise kinship comparisons ranging from 0 to 0.1. The GWAS approach resulted in the identification of 17 and 5 significant associations for seed glucosinolate content and seed hemicellulose content, respectively. The cQTL approach identified 4 significant associations for seed glucosinolate content and 6 significant associations for seed hemicellulose content. The associated SNPs were consistently identified across environments and were mapped to previously reported QTL. These results illustrate the suitability of AM to identify SNP markers associated with seed quality traits in B. napus.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.6
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据