期刊
MOLECULAR BREEDING
卷 35, 期 6, 页码 -出版社
SPRINGER
DOI: 10.1007/s11032-015-0328-z
关键词
Rice; Protein content; qPC-1; Fine mapping
资金
- National Natural Science Foundation [31371233]
- Natural Science Foundation of Jiangsu Province [BK2012684, 14KJA210002]
- project of Six Talent Peaks in Jiangsu Province [2012-NY-027]
- research and innovation program of postgraduate in Jiangsu province [CXLX13_914]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
Protein content (PC) in rice endosperm plays an important role in determining rice grain quality. However, the genetic mechanism underlying grain PC remains unclear. In order to better understand the genetic basis of this trait, a chromosome segment substitution line (CSSL) population derived from the cross of Sasanishiki/Habataki was employed for genetic analysis and gene mapping. In three environments, seven quantitative trait loci in total were identified, of which only qPC-1 was repeatedly detected across three environments, and qPC-10 was identified in two environments; the other five QTLs were detected in one environment. In order to fine-map qPC-1, a CSSL with low PC, SL402, harboring qPC-1, was crossed with Sasanishiki to develop F-2 and F-3 segregation populations. qPC-1 was finally delimited to a 41-kb DNA region on chromosome 1. Storage protein component analysis indicated that the allele from Habataki on qPC-1 can significantly decrease the glutelin content, consequently leading to the decrease in PC. These results provide an important aid for map-based cloning of qPC-1, and the markers linked to qPC-1 could be applied to rice quality improvement.
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