期刊
MOLECULAR BIOLOGY OF THE CELL
卷 26, 期 23, 页码 4248-4264出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E15-08-0603
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资金
- March of Dimes [FY14-247]
- National Institutes of Health [R01GM094415, R01GM083071, T32 CA009156]
- National Science Foundation [IOS0917726]
- Howard Hughes Medical Institute Fellowship of the Helen Hay Whitney Foundation
Eukaryotic cilia are cell-surface projections critical for sensing the extracellular environment. Defects in cilia structure and function result in a broad range of developmental and sensory disorders. However, mechanisms that regulate the microtubule (MT)-based scaffold forming the cilia core are poorly understood. TOG domain array-containing proteins ch-TOG and CLASP are key regulators of cytoplasmic MTs. Whether TOG array proteins also regulate ciliary MTs is unknown. Here we identify the conserved Crescerin protein family as a cilia-specific, TOG array-containing MT regulator. We present the crystal structure of mammalian Crescerin1 TOG2, revealing a canonical TOG fold with conserved tubulin-binding determinants. Crescerin1's TOG domains possess inherent MT-binding activity and promote MT polymerization in vitro. Using Cas9-triggered homologous recombination in Caenorhabditis elegans, we demonstrate that the worm Crescerin family member CHE-12 requires TOG domain-dependent tubulin-binding activity for sensory cilia development. Thus, Crescerin expands the TOG domain array-based MT regulatory paradigm beyond ch-TOG and CLASP, representing a distinct regulator of cilia structure.
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