期刊
MOLECULAR BIOLOGY OF THE CELL
卷 26, 期 4, 页码 685-695出版社
AMER SOC CELL BIOLOGY
DOI: 10.1091/mbc.E14-03-0830
关键词
-
类别
资金
- National Science Foundation [1121710, 1206060]
- National Institutes of Health
- National Cancer Institute
- Center for Cancer Research
- Direct For Biological Sciences
- Div Of Molecular and Cellular Bioscience [1121710] Funding Source: National Science Foundation
T-cells are critical for the adaptive immune response in the body. The binding of the T-cell receptor (TCR) with antigen on the surface of antigen-presenting cells leads to cell spreading and signaling activation. The underlying mechanism of signaling activation is not completely understood. Although cytoskeletal forces have been implicated in this process, the contribution of different cytoskeletal components and their spatial organization are unknown. Here we use traction force microscopy to measure the forces exerted by Jurkat T-cells during TCR activation. Perturbation experiments reveal that these forces are largely due to actin assembly and dynamics, with myosin contractility contributing to the development of force but not its maintenance. We find that Jurkat T-cells are mechanosensitive, with cytoskeletal forces and signaling dynamics both sensitive to the stiffness of the substrate. Our results delineate the cytoskeletal contributions to interfacial forces exerted by T-cells during activation.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据