期刊
MOLECULAR BIOLOGY AND EVOLUTION
卷 32, 期 11, 页码 2818-2831出版社
OXFORD UNIV PRESS
DOI: 10.1093/molbev/msv168
关键词
domestication; hybridization; Saccharomyces eubayanus; lager brewing; genome assembly
资金
- USDA National Institute of Food and Agriculture [1003258]
- National Science Foundation [DEB-1253634]
- DOE Great Lakes Bioenergy Research Center (DOE Office of Science BER) [DE-FC02-07ER64494]
- Project ANPCyT [PICT2011-1814]
- Project UNComahue [B171]
- NSF-CONICET Argentina [5055/14]
- Alexander von Humboldt Foundation
- Pew Charitable Trusts
- Division Of Environmental Biology
- Direct For Biological Sciences [1253634] Funding Source: National Science Foundation
The dramatic phenotypic changes that occur in organisms during domestication leave indelible imprints on their genomes. Although many domesticated plants and animals have been systematically compared with their wild genetic stocks, the molecular and genomic processes underlying fungal domestication have received less attention. Here, we present a nearly complete genome assembly for the recently described yeast species Saccharomyces eubayanus and compare it to the genomes of multiple domesticated alloploid hybrids of S. eubayanus x S. cerevisiae (S. pastorianus syn. S. carlsbergensis), which are used to brew lager-style beers. We find that the S. eubayanus subgenomes of lager-brewing yeasts have experienced increased rates of evolution since hybridization, and that certain genes involved in metabolism may have been particularly affected. Interestingly, the S. eubayanus subgenome underwent an especially strong shift in selection regimes, consistent with more extensive domestication of the S. cerevisiae parent prior to hybridization. In contrast to recent proposals that lager-brewing yeasts were domesticated following a single hybridization event, the radically different neutral site divergences between the subgenomes of the two major lager yeast lineages strongly favor at least two independent origins for the S. cerevisiae x S. eubayanus hybrids that brew lager beers. Our findings demonstrate how this industrially important hybrid has been domesticated along similar evolutionary trajectories on multiple occasions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据