期刊
INTERNATIONAL JOURNAL OF PHARMACEUTICS
卷 448, 期 1, 页码 51-57出版社
ELSEVIER
DOI: 10.1016/j.ijpharm.2013.03.022
关键词
siRNA Mesoporous silica nanoparticles; Delivery; Endosomal escape; Functionalization
资金
- ERC Grant from Science and Technology Commission of Shanghai [11DZ2211000]
- SJTU [AE4160003]
The strategy of encapsulating small interference RNA (siRNA) into the mesopores enables mesoporous silica nanoparticles (MSNs) to be an attractive material for siRNA delivery. Nevertheless, the gene silencing efficiency mediated by this sort of vehicles has not been systematically investigated yet. In this work, through quantifying gene silencing efficiency by flow cytometry (FCM) and performing intracellular tracking by confocal laser scanning microscopy (CLSM), we found that the RNA interference (RNAi) efficiency mediated by MSNs-based delivery vehicles was strongly dependent on their endosomal escape capability. Thereafter, we investigated the correlation between the gene knockdown efficiency and the endosomal escape kinetics of such carriers. The results indicated that highly efficient MSNs-based siRNA delivery vectors must possess the capability of initiating effectively endosomal escape before the degradation of their packaged siRNA in endolysosomes. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据