期刊
MOLECULAR AND CELLULAR PROBES
卷 29, 期 1, 页码 78-80出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2014.12.002
关键词
Tomato black ring virus; Real-time RT-PCR; LNA probe; Detection
类别
资金
- National Science Centre in Poland [DEC-2011/01/N/NZ9/03605, DEC-2013/11/B/NZ9/02510]
Tomato black ring virus (TBRV) infects a wide range of economically important plant species worldwide. In the present study we developed a locked nucleic acid (LNA) real-time RT-PCR assay for accurate detection of genetically diverse TBRV isolates collected from different hosts. The assay based on the LNA probe has a wide detection range, high sensitivity, stability and amplification efficiency. The assay amplified all tested TBRV isolates, but no signal was observed for the RNA from other nepoviruses and healthy plant species. Under optimum reaction conditions, the detection limit was estimated around 17 copies of the TBRV target region in total RNA. Real-time RT-PCR with the LNA probe described in this paper will serve as a valuable tool for robust, sensitive and reliable detection of TBRV isolates. (C) 2014 Elsevier Ltd. All rights reserved.
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