期刊
MOLECULAR AND CELLULAR PROBES
卷 29, 期 2, 页码 92-98出版社
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.mcp.2014.12.001
关键词
HyBeacons; Isothermal amplification; Probes; LAMP; SMAP; CPA
类别
资金
- UK Technology Strategy Board [TP 226-148]
- EPSRC [TS/I000666/1]
- Engineering and Physical Sciences Research Council [TS/I000666/1] Funding Source: researchfish
- EPSRC [TS/I000666/1] Funding Source: UKRI
Isothermal amplification is a rapid, simple alternative to PCR, with amplification commonly detected using fluorescently labelled oligonucleotide probes, intercalating dyes or increased turbidity as a result of magnesium pyrophosphate generation. SNP identification is possible but requires either allele-specific primers or multiple dye-labelled probes, but further downstream processing is often required for allelic identification. Here we demonstrate that modification of common isothermal amplification methods by the addition of HyBeacon probes permits homogeneous sequence detection and discrimination by melting or annealing curve analysis. Furthermore, we demonstrate that isothermal amplification and sequence discrimination is possible directly from a crude sample such as an expressed buccal swab. (C) 2014 Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据