Nano-encapsulations liberated from barley protein microparticles for oral delivery of bioactive compounds

Novel microparticles (3-5 mu m) were created by pre-emulsifying barley proteins with a homogenizer followed a microfluidizer system. These microparticles exhibited a high oil carrying capacity (encapsulation efficiency, 93-97%; loading efficiency, 46-49%). Microparticle degradation and bioactive compound release behaviours were studied in the simulated gastro-intestinal (GI) tract. The data revealed that nano-encapsulations (20-30 nm) were formed as a result of enzymatic degradation of barley protein microparticle bulk matrix in the simulated gastric tract. These nano-encapsulations delivered beta-carotene to a simulated human intestinal tract intact, where they were degraded by pancreatic enzymes and steadily released the beta-carotene. These uniquely structured microparticles may provide a new strategy for the nutraceutical and pharmaceutical industries to develop targeted delivery systems for lipophilic bioactive compounds. (c) 2011 Elsevier B.V. All rights reserved.