期刊
INTERNATIONAL JOURNAL OF PHARMACEUTICS
卷 403, 期 1-2, 页码 292-297出版社
ELSEVIER
DOI: 10.1016/j.ijpharm.2010.10.046
关键词
Zoledronic acid; Self-assembly nanoparticles; Calcium phosphate; Cationic liposomes
资金
- Regione Campania-Laboratori Pubblici Hauteville
- MIUR-PRIN
- Italian Association for Cancer Research (AIRC)
- Ministero della Salute
- Italian Foundation for Cancer Research (FIRC)
Bisphosphonates (BPs) are molecules able to induce apoptosis in several cancer cell lines. However, their short half-life and the rapid uptake and accumulation within bone, limit its use as antitumor agent for extra-skeletal malignancies. Here we proposed a new delivery system to avoid BP accumulation into the bone, thus improving extra-skeletal bioavailability. In this work, we used the zoledronic acid (ZOL), a third generation bisphosphonate, able to induce apoptosis at micromolar concentration. We developed ZOL-containing self-assembly PEGylated nanoparticles (NPs) based on ZOL complexes with calcium phosphate NPs (CaPZ NPs) and cationic liposomes. PEGylation was achieved by two different strategies. CaPZ NPs were covered with PEGylated liposomes (pre-PLCaPZ NPs); alternatively, CaPZ NPs were previously mixed with cationic liposomes and then PEGylated by post-insertion method (post-PLCaPZ NPs). The NPs were fully characterized in terms of mean diameter and size distribution, morphology, ZOL loading, antiproliferative effect on different cell lines. Pre-PLCaPZ NPs showed the best technological characteristics, with a narrow size distribution and a high ZOL loading. Moreover, on different cancer cell lines, these NPs enhanced the antiproliferative effect of ZOL Finally, in an animal model of prostate cancer, a significant reduction of tumor growth was achieved with pre-PLCaPZ NPs, while the tumor was unaffected by ZOL in solution. (C) 2010 Elsevier B.V. All rights reserved.
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