期刊
INTERNATIONAL JOURNAL OF PHARMACEUTICS
卷 400, 期 1-2, 页码 234-242出版社
ELSEVIER
DOI: 10.1016/j.ijpharm.2010.08.040
关键词
Protein delivery system; Anti-inflammatory cytokine; Interleukin-10; Endotoxin-activated macrophages; Dextrin nanogel
资金
- Fundacao para a Ciencia e Tecnologia (FCT), Portugal [SFRH/BD/27359/2006]
- FCT [PTDC/BIO/67160/2006]
- Fundação para a Ciência e a Tecnologia [PTDC/BIO/67160/2006, SFRH/BD/27359/2006] Funding Source: FCT
Interleukin-10 (IL-10) is an anti-inflammatory cytokine, which active form is a non-covalent homodimer with two intramolecular disulphide bonds essential for its biological activity. A mutated form of murine IL-10 was successfully expressed in E. coli, recovered and purified from inclusion bodies. Its ability to reduce tumor necrosis factor alpha synthesis and down-regulate class II major histocompatibility complex molecules expression on endotoxin-stimulated bone marrow-derived macrophages was confirmed, and shown to be similar to that of a commercially available IL-10. Given the potential of IL-10 for application in various medical conditions, it is essential to develop systems that can effectively deliver the protein. In this work it is shown that a dextrin nanogel effectively incorporate IL-10, stabilize, and enable the slow release of biologically active IL-10 over time. Altogether, these results demonstrate the suitability of dextrin nanogel to be used as a system for the controlled release of IL-10. (C) 2010 Elsevier B.V. All rights reserved.
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