期刊
INTERNATIONAL JOURNAL OF PHARMACEUTICS
卷 385, 期 1-2, 页码 194-202出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijpharm.2009.10.041
关键词
Branched PEI (25 kDa); Alginic acid; GFP; Transfection; Cytotoxicity; siRNA
资金
- Department of Biotechnology, New Delhi, India
- CSIR [NWP35]
Branched polyethylenimine (PEI, 25 kDa) was ionically interacted with varying amount of alginic acid to block different proportion (2.6-5.7%) of amines in PEI to form a series of nanocomposites, PEI-Al. These nanocomposites, upon interaction with DNA, protected it against DNase I. Among various complexes evaluated, PEI-Al(4.8%)/DNA displayed the highest transfection efficiency in HEK293, COS-1 and HeLa cells that was similar to 2-8-folds higher than Superfect (TM), Fugene (TM), PEI (750kDa)-Al(6.26%) and PEI alone. The projected nanocomposites were nearly non-toxic to cells in vitro.Furthermore, the concentration of PEI-Al(4.8%) needed to deliver GFP-specific siRNA in COS-1 cells was 20 times lower than PEI (750 kDa)Al(6.26%). Intracellular trafficking of PEI-Al(4.8%) with or without complexed DNA in HeLa cells shows that both appear in the nucleus after 1 h. (c) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据