期刊
INTERNATIONAL JOURNAL OF PHARMACEUTICS
卷 381, 期 2, 页码 106-112出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijpharm.2009.04.037
关键词
Nanoparticle; Antibody; Cytokeratin; Cathepsin; Cystatin; Plasmin
资金
- Research Agency of the Republic of Slovenia [P4-0127]
- Seventh EU Framework IP project NanoPhoto
The primary aim in cancer therapy is to deliver anti-cancer drugs to their specific molecular targets in the tumour. Here we present a system composed of poly(D,L-lactide-co-glycolide) nanoparticles, cytokeratin specific monoclonal antibody and cystatin, a potent protease inhibitor. that can neutralize the excessive proteolytic activity associated with the invasive and metastatic potential of breast turnout cells. The antibody provides specific targeting of the delivery system to invasive breast epithelial cells and, additionally, prevents the generation of plasmin, a central extracellular protease involved in malignant progression. Polymeric nanoparticles rapidly enter the targeted cells and release the inhibitor cargo within the endosomes/lysosomes. The inhibitor is capable to inactivate lysosomal cysteine proteases, in particular cathepsin B, which is involved in the degradation of extracellular matrix inside the turnout cells. Our approach, which combines nanoparticulate delivery system with the inhibitory potential against extracellular and intracellular proteases, may improve the efficacy of therapy in patients with breast tumours compared to the application of individual protease inhibitors. (C) 2009 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据