期刊
INTERNATIONAL JOURNAL OF PHARMACEUTICS
卷 364, 期 1, 页码 94-101出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijpharm.2008.07.027
关键词
Gold nanoparticles; Small interfering RNA (siRNA); Poly(ethylene glycol); Polyelectrolyte complex; Gene delivery
资金
- Ministry of Health and Welfare
- Ministry of Science and Technology
- National Research Foundation of Korea [2006-08301, 2006-02167] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Gold nanoparticles chemically modified with primary amine groups were developed as intracellular delivery vehicles for therapeutic small interfering RNA (siRNA). The positively charged gold nanoparticles could form stable polyelectrolyte complexes through electrostatic interactions with negatively charged siRNA-polyethylene glycol (PEG) conjugates having a cleavable di-sulfide linkage under reductive cytosol condition. The resultant core/shell type polyelectrolyte complexes surrounded by a protective PEG shell layer had a well-dispersed nanostructure with a hydrodynamic diameter of 96.3 +/- 25.9 nm, as determined by dynamic light scattering and transmission electron microscopy. Confocal laser scanning microscopy revealed that the nanosized polyelectrolyte complexes were efficiently internalized in human prostate carcinoma cells, and thus enhanced intracellular uptake of siRNA. Furthermore, the siRNA/gold complexes significantly inhibited the expression of a target gene within the cells without showing severe cytotoxicity. The current Study demonstrated that positively charged gold nanoparticles could be potentially applied for intracellular delivery of siRNA. (C) 2008 Elsevier B.V. All rights reserved.
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