期刊
INTERNATIONAL JOURNAL OF PHARMACEUTICS
卷 353, 期 1-2, 页码 233-242出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijpharm.2007.11.037
关键词
niosomes; bola-surfactant; 5-fluorouracil; percutaneous permeation; cell cytotoxicity
An innovative niosomal system made up of alpha,omega-hexadecyl-bis-(1-aza-18-crown-6) (Bola), Span 801 and cholesterol (2:5:2 molar ratio) was proposed as a topical delivery system for 5-fluorouracil (5-FU), largely used in the treatment of different forms of skin cancers. Bola-niosomes showed a mean size of similar to 400 nm, which were reduced to similar to 200 nm by a sonication procedure with a polydispersion index value of 0.1. Bolaniosomes showed a loading capacity of similar to 40% with respect to the amount of 5-FU added during the preparation. 5-FU-loaded bola-niosomes were tested on SKMEL-28 (human melanoma) and HaCaT (non-melanoma skin cancer with a specific mutations in the p53 tumor suppressor gene) to assess the cytotoxic activity with respect to the free drug. 5-FU-Ioaded bola-niosomes showed an improvement of the cytotoxic effect with respect to the free drug. Confocal laser scanning microscopy studies were carried out to evaluate both the extent and the time-dependent bola-niosome-cell interaction. The percutaneous permeation of 5-FU-Ioaded niosomes was evaluated by using human stratum corneum and epidermis membranes. Bola-niosomes provided an increase of the drug penetration of 8- and 4-folds with respect to a drug aqueous solution and to a mixture of empty bola-niosomes with a drug aqueous solution. 0 2007 Elsevier B.V. All rights reserved.
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