期刊
MOLECULAR AND CELLULAR ENDOCRINOLOGY
卷 414, 期 C, 页码 91-98出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.mce.2015.07.024
关键词
Androgen receptor; Molecular apocrine breast cancer cell; Gene expression; Chromatin binding; ChIP-seq; PIAS1
资金
- Academy of Finland
- Sigrid Juselius Foundation
- Finnish Cancer Organisations
- University of Eastern Finland
We have analyzed androgen receptor (AR) chromatin binding sites (ARBs) and androgen-regulated transcriptome in estrogen receptor negative molecular apocrine breast cancer cells. These analyses revealed that 42% of ARBs and 39% androgen-regulated transcripts in MDA-MB453 cells have counterparts in VCaP prostate cancer cells. Pathway analyses showed a similar enrichment of molecular and cellular functions among AR targets in both breast and prostate cancer cells, with cellular growth and proliferation being among the most enriched functions. Silencing of the coregulator SUMO ligase PIAS1 in MDA-MB453 cells influenced AR function in a target-selective fashion. An anti-apoptotic effect of the silencing suggests involvement of the PIAS1 in the regulation of cell death and survival pathways. In sum, apocrine breast cancer and prostate cancer cells share a core AR cistrome and target gene signature linked to cancer cell growth, and PIAS1 plays a similar coregulatory role for AR in both cancer cell types. (C) 2015 Elsevier Ireland Ltd. All rights reserved.
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