期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 35, 期 7, 页码 1269-1280出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.01118-14
关键词
-
资金
- Grants-in-Aid for Scientific Research [25660055] Funding Source: KAKEN
Methylglyoxal is a typical 2-oxoaldehyde derived from glycolysis. We show here that methylglyoxal activates the Pkc1-Mpk1 mitogen-activated protein (MAP) kinase cascade in a target of rapamycin complex 2 (TORC2)-dependent manner in the budding yeast Saccharomyces cerevisiae. We demonstrate that TORC2 phosphorylates Pkc1 at Thr1(125) and Ser1(143). Methylglyoxal enhanced the phosphorylation of Pkc1 at Ser1(143), which transmitted the signal to the downstream Mpk1 MAP kinase cascade. We found that the phosphorylation status of Pkc1T1(125) affected the phosphorylation of Pkc1 at Ser1(143), in addition to its protein levels. Methylglyoxal activated mammalian TORC2 signaling, which, in turn, phosphorylated Akt at Ser(473). Our results suggest that methylglyoxal is a conserved initiator of TORC2 signaling among eukaryotes.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据