期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 35, 期 8, 页码 1401-1413出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.00112-15
关键词
-
资金
- National Institute of Neurological Disorders and Stroke [R01NS059876, R01NS078402]
- National Cancer Institute [P50CA127001]
- Cancer Prevention and Research Institute of Texas [RP140411]
- National Institute of General Medicine [R01GM099837]
- American Heart Association [AHA0325791T]
Directional cell motility is essential for normal development and physiology, although how motile cells spatiotemporally activate signaling events remains largely unknown. Here, we have characterized an adhesion and signaling unit comprised of protein tyrosine phosphatase (PTP)-PEST and the extracellular matrix (ECM) adhesion receptor beta 8 integrin that plays essential roles in directional cell motility. beta 8 integrin and PTP-PEST form protein complexes at the leading edge of migrating cells and balance patterns of Rac1 and Cdc42 signaling by controlling the subcellular localization and phosphorylation status of Rho GDP dissociation inhibitor 1 (RhoGDI1). Translocation of Src-phosphorylated RhoGDI1 to the cell's leading edge promotes local activation of Rac1 and Cdc42, whereas dephosphorylation of RhoGDI1 by integrin-bound PTP-PEST promotes RhoGDI1 release from the membrane and sequestration of inactive Rac1/Cdc42 in the cytoplasm. Collectively, these data reveal a finely tuned regulatory mechanism for controlling signaling events at the leading edge of directionally migrating cells.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据