期刊
INTERNATIONAL JOURNAL OF ONCOLOGY
卷 45, 期 1, 页码 113-120出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijo.2014.2391
关键词
pancreatic cancer; myofibroblast; peritoneal dissemination; cancer-stromal interaction
类别
资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [24390318, 25670586, 24390319, 25670585, 23390327, 25670584, 25293285, 25670582]
- Grants-in-Aid for Scientific Research [24791431, 25861198, 24591015, 23390327, 25670584, 26293305, 25461024, 26462063, 24591014, 25293285, 25670585, 24592031, 24390319, 24592032, 25670586, 24390318, 25670582] Funding Source: KAKEN
Myofibroblasts in the stroma of pancreatic cancers promote tumor proliferation, invasion and metastasis by increasing extracellular matrix and secretion of several growth factors. In contrast, the role of myofibroblasts at peritoneally disseminated sites of pancreatic cancer has not yet been determined. This study was designed to assess the role of myofibroblasts at peritoneally disseminated sites of pancreatic cancer. Three primary cultures of human peritoneal myofibroblasts (hPMFs) were established from disseminated sites of pancreatic cancer and their interactions with the SUIT-2 and CAPAN-1 human pancreatic cancer cell lines were analyzed in vitro. Using a model in BALB/c nu/nu mice, we compared the dissemination ability of intraperitoneally implanted pancreatic cancer cells, with and without hPMFs, and examined the presence of green fluorescent protein (GFP)-labeled hPMFs at peritoneally disseminated sites in mice. hPMFs significantly promoted the migration and invasion of pancreatic cancer cells (P<0.05), while the cancer cells significantly promoted the migration and invasion of hPMFs (P<0.05). In vivo, the number of peritoneally disseminated nodules, more than 3 mm in size, was significantly greater in mice implanted with cancer cells plus hPMFs compared to mice implanted with cancer cells alone, with GFP-labeled hPMFs surviving in the peritoneal cavity of the former. hPMFs promote the peritoneal dissemination of pancreatic cancer. The cancer-stromal cell interaction in the peritoneal cavity may be a new therapeutic target to prevent the dissemination of pancreatic cancer.
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