期刊
INTERNATIONAL JOURNAL OF ONCOLOGY
卷 42, 期 4, 页码 1257-1262出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijo.2013.1803
关键词
ovarian cancer; biomarker; Experion; matrix-assisted laser desorption/ionization time-of-flight
类别
资金
- National Research Foundation of Korea (NRF)
- Ministry of Education, Science and Technology [2012-000-8766]
Ovarian cancer is the most common cause of disease-related death in women globally. Detection of ovarian cancer using new biomarkers is necessary for early diagnosis. To date, there have been no obvious biomarkers for ovarian cancer detection in the incipient stage. In this study, we discovered potential diagnostic serological biomarkers for ovarian cancer using the Experion (TM) automated electrophoresis system. Sera from 14 healthy women and 84 ovarian cancer patients at stages I- IV were applied to the Experion to compare the protein expression levels. To examine the protein expression pattern of Experion data, proteins in the samples were resolved using 10 and 15% sodium dodecyl sulfate-polyacrylamide gel electrophoresis and visualized by silver staining. The candidate biomarkers elevated in ovarian cancer were purified and determined using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. alpha-2-macroglobulin (173.7 kDa), ceruloplasmin (147 kDa), inter-alpha-trypsin inhibitor family heavy chain-related protein (126 kDa), C-1 inhibitor (115.2 kDa) and hemoglobin alpha/beta (14.4 kDa were overexpressed in the ovarian cancer sera. This study documents a novel way to measure ovarian cancer or cancer-related proteins for biomarkers using the Experion assay system, which should be easily adaptable for high-throughput diagnosis to establish databases of ovarian cancer for clinical applications.
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