期刊
INTERNATIONAL JOURNAL OF ONCOLOGY
卷 43, 期 2, 页码 566-574出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijo.2013.1969
关键词
ghrelin receptor gene; antisense transcript; long non-coding RNA; lung cancer; cell migration; non-small cell lung carcinoma
类别
资金
- National Health and Medical Research Council (NHMRC)
- National Breast Cancer Foundation
- Cancer Council Queensland
- Queensland University of Technology (QUT)
The molecular mechanisms involved in non-small cell lung cancer tumourigenesis are largely unknown; however, recent studies have suggested that long non-coding RNAs (lncRNAs) are likely to play a role. In this study, we used public databases to identify an mRNA-like, candidate long non-coding RNA, GHSROS (GHSR opposite strand), transcribed from the antisense strand of the ghrelin receptor gene, growth hormone secretagogue receptor (GHSR). Quantitative real-time RT-PCR revealed higher expression of GHSROS in lung cancer tissue compared to adjacent, non-tumour lung tissue. In common with many long non-coding RNAs, GHSROS is 5' capped and 3' polyadenylated (mRNA-like), lacks an extensive open reading frame and harbours a transposable element. Engineered overexpression of GHSROS stimulated cell migration in the A549 and NCI-H1299 non-small cell lung cancer cell lines, but suppressed cell migration in the Beas-2B normal lung-derived bronchoepithelial cell line. This suggests that GHSROS function may be dependent on the oncogenic context. The identification of GHSROS, which is expressed in lung cancer and stimulates cell migration in lung cancer cell lines, contributes to the growing number of non-coding RNAs that play a role in the regulation of tumourigenesis and metastatic cancer progression.
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