期刊
INTERNATIONAL JOURNAL OF ONCOLOGY
卷 37, 期 2, 页码 317-325出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/ijo_00000680
关键词
cancer associated fibroblast; MAP kinase; migration activity
类别
资金
- Ministry of Health, Labour and Welfare [19-10]
- Grant for Scientific Research Expenses for Health Labour and Welfare Programs
- Foundation for the Promotion of Cancer Research
- 3rd-Term Comprehensive 10-year Strategy for Cancer Control
- Ministry of Education, Culture, Sports, Science and Technology, the Japanese Government
- Grants-in-Aid for Scientific Research [22790365] Funding Source: KAKEN
It is now clear that the association between cancer cells and recruited fibroblasts (cancer-associated fibroblasts; CAFs) leads to alteration of the biological properties of both types of cells and creates a specific microenvironment. Here we report a novel biological property of CAFs and its cellular mechanism using in vivo and in vitro model. Cultured CAFs derived from human lung cancer tissue displayed significantly higher migration activity in response to PDGF-BB than that of fibroblasts from corresponding non-cancerous tissue (NCAFs). Moreover, KM104(GFP) (GFP-labeled human fibroblast cell line) co-cultured with human cancer cell line Capan-1 showed significantly higher migration activity than KM104(GFP) alone. No such phenomenon occurred when KM104(GFP) and Capan-1 were cultured separately. Even after KM104(GFP) were sorted from co-cultured Capan-1, KM104(GFP) retained their enhanced migration activity until passage-5 of culture in the absence of cancer cells. Despite a similar level of phosphorylation of ERK1/2 after exposure to PDGF-BB, the inhibitory effect of MEK inhibitor was significantly higher on migration of KM104(GFP) that had been sorted from co-cultured Capan-1 than of KM104(GFP) alone. This higher dependence on ERK1/2 signaling for cell migration was also seen in CAFs obtained from cancer tissue. The results of this study indicate that by association with cancer cells, CAFs can acquire enhanced migration activity which could be kept after separation from cancer cells and suggest the possibility that higher dependence on ERK1/2 signaling for enhanced migration activity would be one of the biological properties of CAFs.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据