期刊
INTERNATIONAL JOURNAL OF OBESITY
卷 33, 期 2, 页码 219-225出版社
NATURE PUBLISHING GROUP
DOI: 10.1038/ijo.2008.262
关键词
overweight; endothelial progenitor cells; colony-forming units
资金
- National Institutes of Health [HL076434, HL077450, RR00051]
- American Heart Association [0555678Z]
- American Diabetes Association Clinical Research Award
Objective: To investigate whether adiposity influences endothelial progenitor cell (EPC) number and colony-forming capacity. Design: Cross-sectional study of normal weight, overweight and obese adult humans. Participants: Sixty-seven sedentary adults (aged 45-65 years): 25 normal weight (body mass index (BMI) <= 25 kg/m(2); 12 males/13 females); 18 overweight (BMI 25-29.9 kg/m(2); 12 males/6 females); and 24 obese (BMI >= 30 kg/m(2); 18 males/6 females). All participants were non-smokers and free of overt cardiometabolic disease. Measurements: Peripheral blood samples were collected and circulating EPC number was assessed by flow cytometry. Putative EPCs were defined as CD45(-)/CD34(+)/VEGFR-2(+)/CD133(+) or CD45(-)/CD34(+) cells. EPC colony-forming capacity was measured in vitro using a colony-forming unit (CFU) assay. Results: Number of circulating putative EPCs (either CD45(-)/CD34(+)/VEGFR-2(+)/CD133(+) or CD45(-)/CD34(+) cells) was lower (P<0.05) in obese (0.0007 +/- 0.0001%; 0.050 +/- 0.006%) compared with overweight (0.0016 +/- 0.0004%; 0.089 +/- 0.019%) and normal weight (0.0015 +/- 0.0003%; 0.082 +/- 0.008%) adults. There were no differences in EPC number between the overweight and normal weight groups. EPC colony formation was significantly less in the obese (6 +/- 1) and overweight (4 +/- 1) compared with normal weight (9 +/- 2) adults. Conclusion: These results indicate that: (1) the number of circulating EPCs is lower in obese compared with overweight and normal weight adults; and (2) EPC colony-forming capacity is blunted in overweight and obese adults compared with normal weight adults. Impairments in EPC number and function may contribute to adiposity-related cardiovascular risk.
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