期刊
INTERNATIONAL JOURNAL OF NANOTECHNOLOGY
卷 8, 期 8-9, 页码 736-748出版社
INDERSCIENCE ENTERPRISES LTD
DOI: 10.1504/IJNT.2011.041442
关键词
physicochemical; plasmid DNA; non-viral vector; drug delivery
We aim to characterise in vitro physiochemical behaviour of DNA nanoparticles that have been developed by our research group based on the biodegradable natural polymers with metal coordination. A polysaccharide, pullulan, was chosen as the vector of plasmid DNA. Diethylenetriamine pentaacetic acid (DTPA) was chemically introduced to the hydroxyl groups of dextran to obtain pullulan-DTPA. When Zn2+ solution was added to the mixture of pullulan-DTPA and a plasmid DNA, DNA nanoparticles were formed. The conjugation of pullulan-DTPA with Zn2+ coordination decreased the apparent size of plasmid DNA. A negative zeta potential of plasmid DNA became almost 0 mV by the Zn2+-coordinated conjugation with the pullulan-DTPA. The stability of DNA nanoparticles formed through metal coordination depended on the percentage of chelate residues introduced, the mixing weight ratio of the plasmid DNA/DTPA residue used for conjugate preparation. We conclude that physicochemical characterisation of non-viral gene vectors is important to enhance gene expression whereas the use of plasmid DNA is not effective.
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