期刊
INTERNATIONAL JOURNAL OF NANOMEDICINE
卷 9, 期 -, 页码 2335-2344出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S61375
关键词
electrospinning; gelatin; collagen; polycaprolactone; poly(l-lactic acid-co-epsilon-caprolactone)
资金
- National Natural Science Fund of China [31200735, 81271726, 80170151]
- Shanghai Science and Technology Project [13ZR1426400, 134119a0400]
- Shanghai Natural Science Fund for Youth Scholars [12ZR1446500]
- Science and Technology Development Fund of Shanghai Pudong [PKJ2011-Y320, PKJ-Y30]
- Biomedical Engineering Fund of Shanghai Jiao Tong University [YG2012MS36, YG2012MS35, YG2011ZD03]
- College Young Teachers' Training and Funding Project of Shanghai [ZZjdyx12117, ZZjdyx12124]
- College Young Teachers' Training and Funding Project of Shanghai Jiao Tong University School of Medicine
Electrospun hybrid nanofibers prepared using combinations of natural and synthetic polymers have been widely investigated in cardiovascular tissue engineering. In this study, electrospun gelatin/polycaprolactone (PCL) and collagen/poly(l-lactic acid-co-epsilon-caprolactone) (PLCL) scaffolds were successfully produced. Scanning electron micrographs showed that fibers of both membranes were smooth and homogeneous. Water contact angle measurements further demonstrated that both scaffolds were hydrophilic. To determine cell attachment and migration on the scaffolds, both hybrid scaffolds were seeded with human umbilical arterial smooth muscle cells. Scanning electron micrographs and MTT assays showed that the cells grew and proliferated well on both hybrid scaffolds. Gross observation of the transplanted scaffolds revealed that the engineered collagen/PLCL scaffolds were smoother and brighter than the gelatin/PCL scaffolds. Hematoxylin and eosin staining showed that the engineered blood vessels constructed by collagen/PLCL electrospun membranes formed relatively homogenous vessel-like tissues. Interestingly, Young's modulus for the engineered collagen/PLCL scaffolds was greater than for the gelatin/PCL scaffolds. Together, these results indicate that nanofibrous collagen/PLCL membranes with favorable mechanical and biological properties may be a desirable scaffold for vascular tissue engineering.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据