4.7 Article

Preparation and characterization of Tamoxifen citrate loaded nanoparticles for breast cancer therapy

期刊

INTERNATIONAL JOURNAL OF NANOMEDICINE
卷 9, 期 -, 页码 3107-3118

出版社

DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S63535

关键词

polylactide-co-glycolide nanoparticle; PLGA; breast cancer; multiple emulsion

资金

  1. Indian Council of Medical Research [45/20/2010-NAN/BMS]

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Background: Four formulations of Tamoxifen citrate loaded polylactide-co-glycolide (PLGA) based nanoparticles (TNPs) were developed and characterized. Their internalization by Michigan Cancer Foundation-7 (MCF-7) breast cancer cells was also investigated. Methods: Nanoparticles were prepared by a multiple emulsion solvent evaporation method. Then the following studies were carried out: drug-excipients interaction using Fourier transform infrared spectroscopy (FTIR), surface morphology by field emission scanning electron microscopy (FESEM), zeta potential and size distribution using a Zetasizer Nano ZS90 and particle size analyzer, and in vitro drug release. In vitro cellular uptake of nanoparticles was assessed by confocal microscopy and their cell viability (%) was studied. Results: No chemical interaction was observed between the drug and the selected excipients. TNPs had a smooth surface, and a nanosize range (250-380 nm) with a negative surface charge. Drug loadings of the prepared particles were 1.5%+/- 0.02% weight/weight (w/w), 2.68%+/- 0.5% w/w, 4.09%+/- 0.2% w/w, 27.16%+/- 2.08% w/w for NP1-NP4, respectively. A sustained drug release pattern from the nanoparticles was observed for the entire period of study, ie, up to 60 days. Further, nanoparticles were internalized well by the MCF-7 breast cancer cells on a concentration dependent manner and were present in the cytoplasm. The nucleus was free from nanoparticle entry. Drug loaded nanoparticles were found to be more cytotoxic than the free drug. Conclusion: TNPs (NP4) showed the highest drug loading, released the drug in a sustained manner for a prolonged period of time and were taken up well by the MCF-7 breast cancer cell line in vitro. Thus the formulation may be suitable for breast cancer treatment due to the good permeation of the formulation into the breast cancer cells.

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