期刊
INTERNATIONAL JOURNAL OF NANOMEDICINE
卷 9, 期 -, 页码 2767-2778出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S60171
关键词
alginate; mesoporous silica nanoparticles; atomization; sustained release; targeting therapy
资金
- National Science Council of Taiwan [101-2628-E-002-015-MY3]
- National Taiwan University [102R7842, 102R7740]
- National Health Research Institute [03A1-BNMP14-014]
A new microsphere consisting of inorganic mesoporous silica nanoparticles (MSNs) and organic alginate ( denoted as MSN@Alg) was successfully synthesized by air-dynamic atomization and applied to the intracellular drug delivery systems (DDS) of liver cancer cells with sustained release and specific targeting properties. MSN@Alg microspheres have the advantages of MSN and alginate, where MSN provides a large surface area for high drug loading and alginate provides excellent biocompatibility and COOH functionality for specific targeting. Rhodamine 6G was used as a model drug, and the sustained release behavior of the rhodamine 6G-loaded MSN@Alg microspheres can be prolonged up to 20 days. For targeting therapy, the anticancer drug doxorubicin was loaded into MSN@Alg microspheres, and the ( lysine) 4-tyrosine-arginine-glycine-aspartic acid (K(4)YRGD) peptide was functionalized onto the surface of MSN@Alg for targeting liver cancer cells, hepatocellular carcinoma (HepG2). The results of the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay and confocal laser scanning microscopy indicate that the MSN@Alg microspheres were successfully uptaken by HepG2 without apparent cytotoxicity. In addition, the intracellular drug delivery efficiency was greatly enhanced (ie, 3.5-fold) for the arginine-glycine-aspartic acid (RGD)-labeled, doxorubicin-loaded MSN@Alg drug delivery system compared with the non-RGD case. The synthesized MSN@Alg microspheres show great potential as drug vehicles with high biocompatibility, sustained release, and targeting features for future intracellular DDS.
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