期刊
INTERNATIONAL JOURNAL OF NANOMEDICINE
卷 7, 期 -, 页码 4353-4362出版社
DOVE MEDICAL PRESS LTD
DOI: 10.2147/IJN.S31365
关键词
protein nanocages; drug delivery system; hepatocyte cell lines specific; hepatitis B virus
资金
- Ministry of Health Labor
- Special Coordination Funds for Promoting Science and Technology (SCF funding program Innovation Center for Medical Redox Navigation), Japan
- Grants-in-Aid for Scientific Research [23700561, 23650288] Funding Source: KAKEN
Protein nanocages are self-organized complexes of oligomers whose three-dimensional architecture can been determined in detail. These structures possess nanoscale inner cavities into which a variety of molecules, including therapeutic or diagnostic agents, can be encapsulated. These properties yield these particles suitable for a new class of drug delivery carrier, or as a bioimaging reagent that might respond to biochemical signals in many different cellular processes. We report here the design, synthesis, and biological characterization of a hepatocyte-specific nanocage carrying small heat-shock protein. These nanoscale protein cages, with a targeting peptide composed of a preS1 derivative from the hepatitis B virus on their surfaces, were prepared by genetic engineering techniques. PreS1-carrying nanocages showed lower cytotoxicity and significantly higher specificity for human hepatocyte cell lines than other cell lines in vitro. These results suggested that small heat-shock protein-based nanocages present great potential for the development of effective targeted delivery of various agents to specific cells.
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