期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 19, 期 10, 页码 -出版社
MDPI
DOI: 10.3390/ijms19102924
关键词
RNA-seq; nodule; P deficiency; symbiotic nitrogen fixation; soybean
资金
- National Natural Science Foundation of China [31872164, 31422046, 31372119]
- National Key Research and Development Program of China [2016YFD0100700]
- Nature Science Foundation of Guangdong Province [2015A030306034]
- High Level Talents Special Support Plan of Guangdong Province [2015TX01N042, 2015TQ01N078]
- Research Team Project of the Natural Science Foundation of Guangdong Province [2016A030312009]
Phosphorus (P) deficiency is a major limitation for legume crop production. Although overall adaptations of plant roots to P deficiency have been extensively studied, only fragmentary information is available in regard to root nodule responses to P deficiency. In this study, genome wide transcriptome analysis was conducted using RNA-seq analysis in soybean nodules grown under P-sufficient (500 M KH2PO4) and P-deficient (25 M KH2PO4) conditions to investigate molecular mechanisms underlying soybean (Glycine max) nodule adaptation to phosphate (Pi) starvation. Phosphorus deficiency significantly decreased soybean nodule growth and nitrogenase activity. Nodule Pi concentrations declined by 49% in response to P deficiency, but this was well below the 87% and 88% decreases observed in shoots and roots, respectively. Nodule transcript profiling revealed that a total of 2055 genes exhibited differential expression patterns between Pi sufficient and deficient conditions. A set of (differentially expressed genes) DEGs appeared to be involved in maintaining Pi homeostasis in soybean nodules, including eight Pi transporters (PTs), eight genes coding proteins containing the SYG1/PHO81/XPR1 domain (SPXs), and 16 purple acid phosphatases (PAPs). The results suggest that a complex transcriptional regulatory network participates in soybean nodule adaption to Pi starvation, most notable a Pi signaling pathway, are involved in maintaining Pi homeostasis in nodules.
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