期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 19, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/ijms19092809
关键词
mucin; microbiota; colitis; carcinogenesis; colorectal cancer
资金
- Key Scientific Research Projects for Higher Education of Henan Province [17A310022]
- Scientific Research and Cultivating Fund of Xinxiang Medical University [2013ZD102]
- Innovation Team of Science and Technology, Henan Province
- Key Laboratory of Precision Oncology of Shandong Higher Education, Shandong, China
Gut dysbiosis is associated with colitis-associated colorectal carcinogenesis, and the genetic deficiency of the Muc2 gene causes spontaneous development of colitis and colorectal cancer. Whether there are changes of gut microbiota and a linkage between the changes of microbiota and intestinal pathology in Muc2(-/-) mice are unclear. Muc2(-/-) and Muc2(+/+) mice were generated by backcrossing from Muc2(+/-) mice, and the fecal samples were collected at different dates (48th, 98th, 118th, 138th, and 178th day). Gut microbiota were analyzed by high-throughput sequencing with the universal 16S rRNA primers (V3-V5 region). All mice were sacrificed at day 178 to collect colonic tissue and epithelial cells for the analysis of histopathology and inflammatory cytokines. On the 178th day, Muc2(-/-) mice developed colorectal chronic colitis, hyperplasia, adenomas and adenocarcinomas, and inflammatory cytokines (e.g., cyclooxygenase 2 (COX-2), interleukin 6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), interleukin 1 beta (IL-1 beta), i-kappa-B-kinase beta (IKK beta)) were significantly increased in colonic epithelial cells of Muc2(-/-) mice. In general, structural segregation of gut microbiota was observed throughout the experimental time points between the Muc2(-/-) and Muc2(+/+) mice. Impressively, in Muc2(-/-) mice, Alpha diversities reflected by Shannon and Chao indexes were higher, the phylum of Firmicutes was enriched and Bacteroidetes was decreased, and Desulfovibrio, Escherichia, Akkermansia, Turicibacter, and Erysipelotrichaceae were significantly increased, but Lactobacilli and Lachnospiraceae were significantly decreased. Moreover, the abundance of Ruminococcaceae and butyrate-producing bacteria was significantly higher in the Muc2(-/-) mice. There were significant differences of gut microbiota between Muc2(-/-) and Muc2(+/+) mice. The dynamic changes of microbiota might contribute to the development of colitis and colitis-associated colorectal carcinogenesis. Therefore, this study revealed specific functional bacteria in the development of colitis and colitis-associated colorectal carcinogenesis, which will benefit the development of preventive and therapeutic strategies for chronic inflammation and its malignant transformation.
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