期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 19, 期 9, 页码 -出版社
MDPI
DOI: 10.3390/ijms19092796
关键词
i-motif; CCG repeats; trinucleotide repeat DNA; chromomycin A3; neurological disease; X-ray crystallography; nucleotide flip-out; DNA deformation
资金
- Ministry of Science and Technology, Taiwan [106-2628-M-005-001-MY3]
- Taichung Veterans General Hospital/National Chung Hsing University Joint Research Program [TCVGH-NCHU10776010]
We have reported the propensity of a DNA sequence containing CCG repeats to form a stable i-motif tetraplex structure in the absence of ligands. Here we show that an i-motif DNA sequence may transition to a base-extruded duplex structure with a GGCC tetranucleotide tract when bound to the (Co-II)-mediated dimer of chromomycin A3, Co-II(Chro)(2). Biophysical experiments reveal that CCG trinucleotide repeats provide favorable binding sites for Co-II(Chro)(2). In addition, water hydration and divalent metal ion (Co-II) interactions also play a crucial role in the stabilization of CCG trinucleotide repeats (TNRs). Our data furnish useful structural information for the design of novel therapeutic strategies to treat neurological diseases caused by repeat expansions.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据