期刊
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
卷 15, 期 10, 页码 18840-18855出版社
MDPI AG
DOI: 10.3390/ijms151018840
关键词
fucoidan; osteoclastogenesis; sulfated polysaccharide; bone resorption; RANKL (receptor activator of nuclear factor kappa B (NF-kappa B) ligand)
资金
- Kyungpook National University
Multinucleated osteoclasts differentiate from hematopoietic progenitors of the monocyte/macrophage lineage. Because of its pivotal role in bone resorption, regulation of osteoclast differentiation is a potential therapeutic approach to the treatment of erosive bone disease. In this study, we have found that fucoidan, a sulfated polysaccharide extracted from brown seaweed, inhibited osteoclast differentiation. In particular, addition of fucoidan into the early stage osteoclast cultures significantly inhibited receptor activator of nuclear factor kappa B (NF-kappa B) ligand (RANKL)-induced osteoclast formation, thus suggesting that fucoidan affects osteoclast progenitors. Furthermore, fucoidan significantly inhibited the activation of RANKL-dependent mitogen-activated protein kinases (MAPKs) such as JNK, ERK, and p38, and also c-Fos and NFATc1, which are crucial transcription factors for osteoclastogenesis. In addition, the activation of NF-kappa B, which is an upstream transcription factor modulating NFATc1 expression, was alleviated in the fucoidan-treated cells. These results collectively suggest that fucoidan inhibits osteoclastogenesis from bone marrow macrophages by inhibiting RANKL-induced p38, JNK, ERK and NF-kappa B activation, and by downregulating the expression of genes that partake in both osteoclast differentiation and resorption.
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